王倩 教授 博士生导师
电话：0532-58631580
E-mail：qiqi20011983@gmail.com，qiqi20011983@sdu.edu.cn
山东青岛市滨海大道72号山东大学微生物技术国家重点实验室N1-236
学习和工作经历
2020年-今山东大学国家糖工程技术研究中心教授博士生导师
2016年获选山东大学“青年学者未来计划”人才支持计划
2015-2020年山东大学国家糖工程技术研究中心副教授
博士2004年-2009年山东大学微生物技术国家重点实验室微生物学
学士2000年-2004年山东农业大学生物技术
研究方向
1.利用合成生物学方法对细胞工厂代谢网络进行重新设计、构建和调控，实现高效碳转化效率的人工途径的构建。
2.开发动态调控元件及代谢物生物传感器，用于途径代谢流的精确调节。
3.以微生物为细胞工厂生产生物可降解塑料、生物基化合物及5-氨基乙酰丙酸、N-乙酰神经氨酸等高附加值产品。
主持项目
2019-2024, 人工节碳途径的组装及其与宿主的适配和调控2019国家重点研发计划子课题（2019YFA0904900）
2019-2024, 重组天然的塑料降解酶用于外消旋结晶2019国家重点研发计划子课题（2019YFA0706900）
2017-2020, 谷氨酸棒杆菌双组份系统HrrSA、ChrSA分子调控机制及其对血红素合成调控机制研究，国家自然科学基金面上项目(31670047)；
2017-2018，谷氨酸棒杆菌代谢调控生产5-氨基乙酰丙酸，山东省重点研发计划项目，(2017GSF21121)；
2013-2015，利用基因振荡回路合成PHBV嵌段式共聚物的基础研究，国家自然科学基金青年基金(31200033)；
2012-2015，5-氨基乙酰丙酸工程菌的基因组规模系统代谢育种，国家863计划“工业微生物基因组及分子改造”子课题，（2012AA022104）；
2013-2015，大肠杆菌利用群体感应调控合成PHBV的基础研究，高等学校博士学科点专项科研基金 (20120131120081)；
2012-2014，大肠杆菌以单一碳源生产短链聚羟基脂肪酸酯PHBV的途径构建及其代谢网络研究，山东省优秀中青年科学家奖励基金（BS2012SW005）；
2010-2012，大肠杆菌利用非相关碳源合成PHBV研究，山东大学自主创新基金（2010GN035）。
代表论文
1.Jian Zhang, Zhiguo Wang, Tianyuan Su, Huanhuan Sun, Yuan Zhu, Qingsheng Qi*, QianWang*. Tuning the Binding Affinity of Heme-Responsive Biosensor for Precise and Dynamic Pathway Regulation.iScience. 23, 5, 22 May 2020, 101067.
2.In vivo evolutionary engineering of riboswitch with high-threshold for N-acetylneuraminic acid production.Metabolic Engineering, Metab Eng. 2020 May; 59:36-43.
3.Qingxiao Pang, Hao Han, Ya Xu, Xiaoqin Liu, Qingsheng Qi*, Qian Wang*. Exploring Amino Sugar and Phosphoenolpyruvate Metabolism to ImproveEscherichia coliN-Acetylneuraminic Acid Production.J Agric Food Chem.2020 Oct 21; 68(42):11758-11764.
4.Ying Li, Zhijie Sun, Ya Xu, Yaqi Luan, Jiasheng Xu, Quanfeng Liang, Qingsheng Qi, Qian Wang*. Enhancing the Glucose Flux of an Engineered EP-Bifido Pathway for High Poly(Hydroxybutyrate) Yield Production.Front Bioeng Biotechnol.2020 Aug 27; 8:517336.
5.Xiaoli Yu, Changsen Lin, Jing Yu, Qingsheng Qi*, Qian Wang*. BioengineeredEscherichia coliNissle 1917 for tumour-targeting therapy.Microb Biotechnol.2020 May; 13(3):629-636.
6.Ding Zhou, Zhennan Jiang, Qingxiao Pang, Yuan Zhu,Qian Wang*, Qingsheng Qi, CRISPR/Cas9-Assisted Seamless Genome Editing inLactobacillus plantarumand Its Application in N-Acetylglucosamine Production.Appl. Environ. Microbiol.2019 Aug 23. pii: AEM.01367-19.
7.Tianyuan Su, Qi Guo, Yi Zheng, Quanfeng Liang,Qian Wang* and Qingsheng Qi* Fine-tuning ofhemBusing CRISPRi for increasing 5-aminolevulinic acid production inEscherichia coli.Frontiers in Microbiology.2019 Jul 31; 10:1731.
8.Zhiyong Cui, Zhennan Jiang, Jinhong Zhang, Huihui Zheng, Xin Jiang, Kai Gong, Quanfeng Liang,Qian Wang*, Qingsheng Qi,Stable and Efficient Biosynthesis of 5-Aminolevulinic Acid Using Plasmid-FreeEscherichia coli.J Agric Food Chem.2019, 67, 5, 1478-1483.
9.Qian Wang;Jiasheng Xu ; Zhijie Sun; Yaqi Luan; Ying Li; Junshu Wang; Quanfeng Liang; Qingsheng Qi , Engineering an in vivo EP-bifido pathway in Escherichia coli for high-yield acetyl-CoA generation with low CO2emission.,Metabolic Engineering, 2018.8.10, 51: 79~87.
10.Peng Yang; Jing Wang; Qingxiao Pang; Fengyu Zhang; Junshu Wang;Qian Wang*; Qingsheng Qi*, Pathway optimization and key enzyme evolution of N-acetylneuraminate biosynthesis using an in vivo aptazyme-based biosensor,Metabolic Engineering, 2017.09, 43: 21~28.
11.Peng Yang, Wenjing Liu, Xuelian Cheng, Jing Wang,Qian Wang*, Qingsheng Qi A New Strategy for Production of 5-Aminolevulinic Acid in RecombinantCorynebacterium glutamicumwith High Yield.Appl. Environ. Microbiol82/9/201605/2709-2717.
12.Xiaoli Yu, Haiying Jin, Xuelian Cheng,Qian Wang*, Qingsheng Qi Transcriptomic analysis for elucidating the physiological effects of 5-aminolevulinic acid accumulation onCorynebacterium glutamicum.Microbiological Research192/201611/292–299.
13.Q Wang, Y Luan, X Cheng, Q Zhuang, Q Qi* Engineering ofEscherichia colifor the biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from glucose.Appl Microbiol Biotechnol.2015. 99:2593-2602.
14.Q Zhuang,Q Wang, Q Liang, Q Qi* Synthesis of polyhydroxyalkanoates from glucose that contain medium-chain-length monomers via the reversed fatty acid β-oxidation cycle inEscherichia coli.Metabolic Engineering2014. 24:78-86.
15.Q Wang, X Liu, Q Qi* Biosynthesis of poly(3-hydroxybutyrate- co-3-hydroxyvalerate) from glucose with elevated 3-hydroxyvalerate fraction via combined citramalate and threonine pathway inEscherichia coli.Applied Microbiology and Biotechnology2014.98:3923–3931.DOI 10.1007/s00253-013-5494-5
16.Q Wang, Q Zhuang, Q Liang, Q Qi* Polyhydroxyalkanoic acids from structurally-unrelated carbon sources inEscherichia coli.Applied Microbiology and Biotechnology2013. 97(8): 3301-3307
17.Mingji Li, Pengfei Gu, Junhua Kang, Yang Wang,Qian Wang*, Qingsheng QiExtending homologous sequence based on the single gene mutants by one-step PCR for efficient multiple gene knockouts.Folia Microbiol(Praha).2012 May; 57(3): 209-14. Epub 2012 Mar 30.
18.Q Wang, Y Xia, Q Chen, Q Qi*.Incremental truncation of PHA synthases results in altered product specificity.Enzyme and Microbial Technology.2012 May 10; 50(6-7):293-7. Epub 2012 Feb 25.
19.Q Chen1,Q Wang1, G Wei, Q Liang, and Q Qi*. Production inEscherichia coliof Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) with Differing Monomer Compositions from Unrelate carbon sources.Applied and Environment Microbiology, 2011, July; 77(14):4886-93.
20.Q Wang, H Zhang, Q Chen, X Chen, Y Zhang, Q Qi*. A marine bacterium accumulates polyhydroxyalkanoate consisting of mainly 3-hydroxydodecanoate and 3-hydroxydecanoate.World Journal of Microbiology and Biotechnology. 2010,26:1149–1153.
21.Q Wang, H Yu, Y Xia, Z Kang, Q Qi*. Complete PHB mobilization inEscherichia colienhances the stress tolerance: a potential biotechnological application.Microbial Cell Factories. 2009, 8:47.
授权专利
王倩;刘文静;祁庆生,一种利用重组大肠杆菌厌氧生产5-氨基乙酰丙酸的方法, 2017.4.26,中国, ZL201410448698.3
