Education

Research Experience

Research Interests

Glycosaminoglycans (GAGs), a class of linear polyanionic polysaccharides composed of repeating disaccharides containing hexosamine, are ubiquitously distributed on cell surface and in the extracellular matrix of animal tissues and participate inalmost all physiological and pathological processes. Except nonsulfatedhyaluronan, the other three sulfated GAGs includingchondroitin sulfate/dermatan sulfate, heparin/heparan sulfate and keratan sulfate are synthesized as the side chains of proteoglycans (PGs) and form very complex structures due to various modifications, which presentsgreat challenges to the functionalstudy and application of GAGs/PGs. Research in the Li lab mainly focus on investigating the structure and function of GAGs/PGs by using and developing various tools and methods of biochemistry, molecular biology and cell biology.Current research interests include:

(1) Resource mining, enzymatic characterization, catalytic mechanism research, molecular engineering and application of novel GAG-degrading enzymes (lyases, sulfatases, glycosidases, etc.) from marine and gut microbes;

(2) Study on the structure-function relationship of GAGs from various marine and terrestrial animals and development of related biomedical products;

(3) Development and application of new technologies forin vitro andin vivo analysis and detection of GAGs/PGs by screening and optimizing novel enzymatic tools and biocompatible probes

(4) Study on the role of cell surface PGs in the occurrence and development of cancer and other diseases

Representative Publications

1. Qingdong Zhang, Hai-Yan Cao, Lin Wei, Danrong Lu, Min Du, Min Yuan, Deling Shi, Xiangxue Chen, Peng Wang, Xiu-Lan Chen, Lianli Chi, Yu-zhong Zhang*, and Fuchuan Li*. Discovery of exolytic heparinases and their catalytic mechanism and potential application.Nat. Commun. 2021, 12(1):1263

2. Wenshuang Wang, Nanhan Han, Yingying Xu, Yunxue Zhao, Liran Shi, Jorge Filmus, and Fuchuan Li*. Assembling Custom Side Chains on Proteoglycans to Interrogate Their Function in Living Cells.Nat. Commun. 2020, 11:5915.

3. Chune Peng, Qingbin Wang, Shumin Wang, Wenshuang Wang, Runmiao Jiao, Wenjun Han, and Fuchuan Li*. A chondroitin sulfate and hyaluronic acid lyase with poor activity to glucuronyl 4,6-O-disulfated N-acetylgalactosamine (E-type)-containing structures.J. Biol. Chem. 2018, 293(12):4230-4243.

4. Naihan Han, Wenshuang Wang, Danrong Lu, Han Wang, Xinying Ma, Xiaopeng Fan, and Fuchuan Li*. A novel, rapid, and sensitive homogeneous sandwich detection method of Glypican-3 as a serum marker for hepatocellular carcinoma.Chem. Commun (Camb)., 2017, 53(90):12209-12212.

5. Xiaoru Zhang, Ruijuan Li, Yuanyuan Chen, Shusheng Zhang, Wenshuang Wang, and Fuchuan Li*. Applying DNA rolling circle amplification in fluorescence imaging of cell surface glycans labeled by a metabolic method.Chem. Sci., 2016,7, 6182-6189.

6. Wenshuang Wang, Naihan Han, Ruijuan Li, Wenjun Han, Xiaoru Zhang, and Fuchuan Li*. Supercharged Fluorescent Protein as a Versatile Probe for the Detection of Glycosaminoglycans in Vitro and in Vivo.Anal. Chem. 2015, 87(18):9302-7.

7. Wenshuang Wang, Wenjun Han, Xingya Cai, Xiaoyu Zheng, Kazuyuki Sugahara, and Fuchuan Li*. Cloning and characterization of a novel chondroitin sulfate/dermatan sulfate 4-O-endosulfatase from a marine bacterium. J. Biol. Chem. 2015, 290(12):7823-32.

8. Wenjun Han, Wenshuang Wang, Mei Zhao, Kazuyuki Sugahara, and Fuchuan Li*. A novel eliminase from a marine bacterium that degrades hyaluronan and chondroitin sulfate.J. Biol. Chem. 2014, 289(40):27886-98.

9. Fuchuan Li, Wen Shi,Mariana I Capurro and Jorge Filmus. Glypican-5 stimulatesrhabdomyosarcoma cell proliferation by activating hedgehog signaling. J. Cell Biol., 2011, 192(4):691-704.

10. Fuchuan Li, Chilkunda D. Nandini,Tomohide Hattori,Xingfeng Bao,Daisuke Murayama, Toshikazu Nakamura, Nobuhiro Fukushima,and Kazuyuki Sugahara. Structure of pleiotrophin- and hepatocyte growth factor-binding sulfated hexasaccharide determined by biochemical and computational approaches.J. Biol. Chem.,2010, 285(36):27673-85.

11. Fuchuan Li, Gerdy B. ten Dam, Sengottuvelan Murugan, Shuhei Yamada,Taishi Hashiguchi, Shuji Mizumoto, Kayoko Oguri, Minoru Okayama, Toin H. van Kuppevelt,and Kazuyuki Sugahara. Involvement of Highly Sulfated Chondroitin Sulfate in the Metastasis of the Lewis Lung Carcinoma Cells. J. Biol. Chem., 2008, 283(49):34294-304.

12. Mariana I Capurro, Ping Xu, Wen Shi, Fuchuan Li, Angela Jia and Jorge Filmus. Glypican-3 Inhibits Hedgehog Signaling During Development by Competing with Patched for Hedgehog Binding.Dev. Cell, 2008,14(5):700-11.

13. Fuchuan Li, Ajaya Kumar Shetty and Kazuyuki Sugahara.Neuritogenic activity of chondroitin/dermatan sulfate hybrid chains of embryonic pig brain and their mimicry from shark liver. Involvement of the pleiotrophin and hepatocyte growth factor signaling pathways.J. Biol. Chem., 2007, 282(5): 2956-2966.

Honors and Awards

Taishan Industry Leading Talent of Shandong Province, China

Patents

1. A glycosaminoglycan lyase that is difficult to degrade CS-E and its coding gene and application, Li Fu-chuan, Peng Chun-e, Wang Qing-bin, etc. chinese invention patent, patent number: ZL201710544077.9

2. An exo-type glycosaminoglycan lyase and its coding gene and application, Li Fu-chuan, Cai Xiao-juan, Wang Wen-shuang, etc., chinese invention patent, patent number: ZL201610227396.2

3. A strain of Vibrio FC509 and its cultivation method and application, Li Fu-chuan, Han Wen-jun, Wang Wen-shuang, etc., chinese invention patent, patent number: ZL201410159983.3

4. An endo-type chondroitin sulfate/dermatan sulfate 4-O-sulfatase and its coding gene and application, Li Fu-chuan, Wang Wen-shuang, Zheng Xiao-yu, etc., chinese invention patent, patent number: ZL201410209975.5

5. A glycosaminoglycan-degrading enzyme and its coding gene and application, Li Fu-chuan, Han Wen-jun, Wang Wen-shuang, etc., chinese invention patent, patent number: ZL2014101,60095.3

6. A highly expressed water-soluble heparinase I fusion protein and its coding gene, Li Fu-chuan, Zhao Mei, Han Wen-jun, etc., chinese invention patent, patent number: ZL201410246947.0

7. An exo-type agarase and its coding gene and application, Li Fu-chuan, Han Wen-jun, etc., chinese invention patent, patent number: ZL201410396060.X

8. The application of highly positively charged fluorescent protein in the analysis and detection of glycosaminoglycans, Li Fu-chuan, Wang Wen-shuang, etc., chinese invention patent, patent number: ZL201510044900.0

9. A kit for high-sensitivity detection of proteoglycan and its preparation method, Li Fu-chuan, Han Nai-han, etc., chinese invention patent, patent number: ZL201610143751.8

10. An endo-type algin lyase and its coding gene and application, Li Fu-chuan, Han Wen-jun, etc., chinese invention patent, patent number: ZL2014104698614

11. A high-sulfated chondroitin sulfate and its preparation method and application, Li Fu-chuan, Wang Qing-bin, etc., chinese invention patent, patent number: ZL201610323540.2

12. An exo-type glycosaminoglycan lyase and its coding gene and application, Li Fu-chuan, Wang Wen-shuang, etc., chinese invention patent, patent number: ZL2016102273962