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Sortase A-mediated chemoenzymatic synthesis of complex glycosylphosphatidylinositol-anchored protein

日期:2014-07-28点击:

Zhimeng Wu, Xueqing Guo, Jian Gao and Zhongwu Guo*

Chem. Commun., 2013, 49, 11689-11691

ABSTRACT

Green fluorescent protein and a glycosylphosphatidylinositol (GPI) anchor containing the common core structure and a lipid chain were synthesized and then coupled together in the promotion of bacterial sortase A (SrtA), which was the first example for the synthesis of a full-size GPI-anchored protein by SrtA, demonstrating that this can be a generally useful method for GPI-anchored protein synthesis.