Education

Research Experience

Research Interests

Research on Resource Microbes, polysaccharide-degrading enzymes and Functional Oligosaccharides: (1) Discovering and clarifying new polysaccharide-degrading catalytic mechanisms.(2) Discovering the guiding theories and technical approaches for the transformation of molecular enzymology.

Representative Publications

[1] Yuanyuan Cheng, Qingmin Chen， Junge Li， Shuhuan Li， Yanhui Wang，Wenjun Han*, High Expression and Enzymatic Characterization of an Exo-type Agarase AgaO.Science and Technology of Food Industry, 2019,40(07): 107-113.

[2] Chune Peng, Qingbin Wang, Danrong Lu, Wenjun Han*, Fuchuan Li*. A Novel Bifunctional Endolytic Alginate Lyase with Variable Alginate-Degrading Modes and Versatile Monosaccharide-Producing Properties. Frontiers in Microbiology. 2018, 9:167.

[3] Huihui Liu, Jingyan Gu, Xin Li,Wenjun Han*,Isolation, Identification and Degradation Performance Analysis of Multifunctional Degrading Bacteria for Fragrance Compounds. Chinese scientific papers online, 2017, 10(9):948-953.

[4] Jungang Li, Yuanyuan Cheng, Dandan Wang, Junge Li, Yanhui Wang,Wenjun Han, Fuchuan Li*. Draft Genome Sequence of the Polysaccharide-Degrading Marine BacteriumPseudoalteromonas sp. Strain A601.Genome Announcements. 2017, 5(29):e00590-17.

[5] Yuanyuan Cheng^#, Dandan Wang^#, Jingyan Gu, Junge Li, Huihui Liu, Fuchuan Li, Wenjun Han*. Biochemical Characteristics and Variable Alginate-Degrading Modes of a Novel Bifunctional Endolytic Alginate Lyase. Applied and Environmental Microbiology. 2017, 83(23):e01608-17.

[6] Huihui Liu, Yuanyuan Cheng, Jingyan Gu, Yanhui Wang, Junge Li, Fuchuan Li,Wenjun Han*. Draft Genome Sequence of Paenibacillus sp. Strain MY03, a Terrestrial Bacterium Capable of Degrading Multiple Marine-Derived Polysaccharides. Genome Announcements. 2017, 5(29):e00578-17.

[7] Wenjun Han, Yuanyuan Cheng, Dandan Wang, Shumin Wang, Huihui Liu, Jingyan Gu, Zhihong Wu, Fuchuan Li*. Biochemical Characteristics and Substrate Degradation Pattern of a Novel Exo-Typeβ-Agarase from the Polysaccharide-Degrading Marine Bacterium Flammeovirga sp. Strain MY04. Applied and Environmental Microbiology. 2016, 82(16):4944-4954.

[8] Wenjun Han, Jingyan Gu, Yuanyuan Cheng, Huihui Liu, Yuezhong Li, Fuchuan Li*. Novel Alginate Lyase (Aly5) from a Polysaccharide-Degrading Marine Bacterium Flammeovirga sp. Strain MY04: Effects of Module Truncation to the Biochemical Characteristics, Alginate Degradation Patterns, and Oligosaccharide-Yielding Properties.Applied and EnvironmentalMicrobiology. 2016, 82(1):264-374.

[9]Wenjun Han, Jingyan Gu, Huihui Liu, Fuchuan Li, Zhihong Wu, Yuezhong Li*. Deletion of a non-Catalytic Region Increases the Enzymatic Activity of aβ-Agarase fromFlammeovirgasp. MY04.Journal of Ocean University of China. 2015, 14(5):841-848.

[10]Wenjun Han^#, Wenshuang Wang^#, Mei Zhao, Kazuyuki Sugahara, Fuchuan Li*. A Novel Eliminase from a Marine Bacterium that Degrades Hyaluronan and Chondroitin Sulfate. The Journal of Biological Chemistry. 2014, 289(40):27886-27898.

[11] Wenjun Han^#, Jingyan Gu^#, Hui HuiLiu^#, Fuchuan Li, Zhihong Wu, Yuezhong Li*. An Extra Peptide within the Catalytic Module of aβ-Agarase Affects the Agarose Degradation Pattern. The Journal of Biological Chemistry. 2013, 288(13):9519-9531.

[12]Wenjun Han,Shuai Zhao, Huihui Liu, Zhihong Wu*, Qianqun Gu, Yuezhong Li. Isolation, Identification and Agarose Degradation Ability of a Strain of Polysaccharide Degrading Bacteria,Acta Microbiologica Sinica, 2012, 52(6): 776-

[13]Jingyan Gu,Wenjun Han, Fangfang Qi, Jing Li, Shiwei Sun, Tianjiao Zhu, Wenjun Mao, Qianqun Gu, Dehai Li*. Two new 23-membered macrolactones from a terrestrial bacterium,Streptomycessp. IMBJ01.Helvetica Chimica Acta. 2011, 94: 1448-1453. (shared the first author)

Honors and Awards

1.The executive director of the Interdisciplinary Branch of Shandong Medical Ethics Association. (2021.5.15-2026.5.15)

Patents

. (1)Seaweed polysaccharide-degrading enzyme

[1] National invention patent, Multifunctional polysaccharide degrading enzyme rAly16-1 fromStreptomyces sp. genus and its coding gene and application thereof,patent number: ZL 2021 10379188.5 (The first)

[2] National invention patent,Cladosporium sp. and its application, patent number: ZL 2021 10592534.8 (The first)

[3] National invention patent, APersicobacter sp. JZB09, alginate lyase rAly06925 and coding gene of the enzyme and application, patent number: ZL 2021 10476331.2 (The first)

[4] National invention patent,A disaccharide exo-type β-mannan hydrolase Man02066 and its coding gene and application,patent number: ZL 2020 10525116.2 (The first)

[5] National invention patent,An endo-type β-mannan hydrolase Man01929 and its mutation into glycosyltransferase method and application,patent number: ZL 2020 10307836.1 ((The first) (Authorized)

[6] National invention patent, Monosaccharide exotype alginate lyase Aly-6 having M-tendency, coding gene thereof and use thereof,patent number: ZL 2018 10887656.8, PCT/CN2018/106615 (The first)(Authorized)

[7] National invention patent,A polysaccharide-degrading bacterium of the genusPseudoalteromonas sp. and its culture method and application,patent number: ZL 2018 11528550.5 (The first) (Authorized)

[8] National invention patent,Application of alginate lyase in preparing series of oligosaccharide products,patent number: ZL 2017 10651814.5 (The first) (Authorized)

[9] National invention patent, Arecombinant alginate lyase truncated rAly1-T185N its coding gene andapplication, patent number: ZL 2017 10110627.6 (The first) (Authorized)

[10] National invention patent,A producing multiple monosaccharide products endo-type bifunctional alginate lyase Aly2 and its coding gene,patent number: ZL 2017 114082246 (The first) (Authorized)

[11] National invention patent,Bifunctional endo-recombinant alginate lyase rAly-1 and its coding gene and application thereof,patent number: ZL 2016 10838337.9 (The first) (Authorized)

[12] National invention patent,A polysaccharide degrading bacterium strain ofStreptomycessp. and its culture method and application,patent number: ZL 2016 1 09371025 (The first) (Authorized)

[13] National invention patent，An endo alginate lyase and its coding gene and application，patent number:ZL 2014 10469861.4 (The second) (Authorized)

[14] National invention patent，An exo-agarase and its coding gene and application，patent number:ZL 2014 10396060.X (The second) (Authorized)

[15] National invention patent，Method for preparing oligosaccharide by degrading solid agar，patent number:ZL 2005 10021919.X (The third) (Authorized)

(2)Glycosaminoglycan-degrading enzyme

[1] National invention patent，A hyaluronic acid lyase HyaL16-3 derived from Streptomyces and its coding gene and application,patent number:ZL 2017 10724774.2（The first）(Authorized)

[2]National invention patent，Endo chondroitin sulfate / dermatan sulfate 4-o-sulfatase and its coding gene and application, patent number:ZL 2014 10209975.5 (The fifth) (Authorized)

[3] National invention patent，A highly expressed water-soluble heparanase I fusion protein and its coding gene, patent number: ZL 2014 10246947.0 (The third) (Authorized)

[4] National invention patent，Glycosaminoglycan lyase and its coding gene and application，patent number:ZL 2014 10160095.3 (The second) (Authorized)

[5] National invention patent，AVibriosp. strain FC509 and its culture method and application，patent number:ZL 2014 10159983.3 (The second) (Authorized)

(3)Others

[1] National invention patent, A strain of fragrance bacteria and Its application in the preparation of allyl methyl sulfide, patent number：ZL 2012 10732431.2 (The fourth) (Authorized)

[2] National invention patent, A pyrimidine nucleoside phosphorylase gene ofExiguobacterium sp. MY02 strain and its preparation method, patent number: ZL 2005 10021829.0 (The second) (Authorized)

[3] Utility model patent, A Crushing device for biological mold cake,patent number: ZL 2020 22227297.9 (The first) (Authorized)

[4] Utility model patent, A column chromatography device for purification of recombinant enzyme,patent number: ZL 2020 21889306.4 (The first) (Authorized)

[5] Utility model patent, A low-temperature storage device for recombinase,patent number: ZL 2020 21751290.0 (The first) (Authorized)

[6] Utility model patent, A Constant temperature moisturizing device for preparing recombinant enzyme,patent number: ZL 2020 21751260.X (The first) (Authorized)

[7] Utility model patent, An On-line monitoring device for microbial fermentation tank, patent number: ZL 2020 21691100.0 (The first) (Authorized)

[8] Utility model patent, Abacteria filter device used after microbial fermentation, patent number: ZL 2020 21691096.8 (The first) (Authorized)

[9] Utility model patent, A vacuum freeze-drying device for biological enzyme detection,patent number: ZL 2020 21603346.8 (The first) (Authorized)

[10] Utility model patent, An internal circulation catalytic reactor for biological enzyme,patent number: ZL 2020 21602621.4 (The first) (Authorized)