Education

Research Experience

Research Interests

Microbial physiology and biochemistry,focusing on discovery and molecular evolution of microbial glycoenzymes forenzyme-catalyzed synthesis of oligosaccharides and glycosyl compounds.

Representative Publications

1.Wenzheng Wang, Yajing Sun, Peng Peng, Guofeng Gu, Guoying Du, Li Xu,* andMin Xiao*. Two-Step Enzymatic Conversion of Rebaudioside A into a Mono-α-1,4-Glucosylated Rebaudioside A Derivative. J. Agric. Food Chem. 2021, 69, 2522−2530

2. Peng Liu, Huaqin Zhang, Yuying Wang, Xiaodi Chen, Lan Jin, Li Xu*,Min Xiao*. Screening and characterization of an α-L-fucosidase fromBacteroides fragilis NCTC9343 for synthesis of fucosyl-N-acetylglucosamine disaccharides. Appl Microbiol Biotechnol. 2020, 104: 7827−7840

3. Yinping Li, Weiying Hong, Henan Zhang, Tian Tian Zhang, Zhiyong Chen, Shiling Yuan, Peng Peng*, Min Xiao*, Li Xu*. Photothermally triggered cytosolic drug delivery of glucose functionalized polydopamine nanoparticles in response to tumor microenvironment for the GLUT1-targeting chemo-phototherapy. J Control Release. 2020, 317: 232−245.

4. Xiaodi Chen, Lan Jin, Xukai Jiang, Longcheng Guo, Guofeng Gu , Li Xu 1, Lili Lu, Fengshan Wang,Min Xiao*. Converting a β-N-acetylhexosaminidase into two trans-β-N-acetylhexosaminidases by domain-targeted mutagenesis. Appl Microbiol Biotechnol. 2020, 104(2): 661−673.

5. Li Xu, Xiaohong Liu , Yinping Li , Zhenhao Yin, Lan Jin, Lili Lu, Jingyao Qu,Min Xiao*. Enzymatic rhamnosylation of anticancer drugs by an α-L-rhamnosidase fromAlternaria sp. L1 for cancer-targeting and enzyme-activated prodrug therapy. Appl Microbiol Biotechnol. 2019, 103(19): 7997−8008

6. Ke Wang, Tingting Qi, Longcheng Guo, Zhongxuan Ma, Guofeng Gu,Min Xiao*, Lili Lu*. Enzymatic Glucosylation of Salidroside from Starch by α-Amylase. J Agric Food Chem. 2019, 67, 2012−2019

7. Tiantian Zhang, Yinping Li, Weiying Hong, Zhiyong Chen, Peng Peng, Shiling Yuan, Jingyao Qu,Min Xiao*, Li Xu*. Glucose oxidase and polydopamine functionalized iron oxide nanoparticles: combination of the photothermal effect and reactive oxygen species generation for dual-modality selective cancer therapy. J Mater Chem B. 2019, 7:2190−2200.

8. Chunjuan He, Yougui Huang, Peng Liu, Jianhuan Wei, Yirui Yang, Li Xu*,Min Xiao*. Transcriptome analysis of genes and metabolic pathways associated with nicotine degradation in Aspergillus oryzae 112822. BMC genomics. 2019, 20(1): 86. DOI: 10.1186/s12864-019-5446-2

9. Longcheng Guo, Xiaodi Chen, Li Xu,Min Xiao*, Lili Lu*. Enzymatic Synthesis of 6’-Sialyllactose, a Dominant Sialylated Human Milk Oligosaccharide, by a Novel exo-α-Sialidase fromBacteroides fragilisNCTC9343. Appl Environ Microbiol. 2018, 84(13):e00071-18

10. Chunjuan He, Yirui Yang, Renfei Zhao, Jingyao Qu, Lan Jin, Lili Lu, Li Xu*,Min Xiao*. Rational designed mutagenesis of levansucrase fromBacillus licheniformis8-37-0-1 for product specificity study. Appl Microbiol Biotechnol. 2018, 102(7):3217−3228

11. Tingting Qi, Guofeng Gu, Li Xu,Min Xiao*, Lili Lu*. Efficient synthesis of tyrosol galactosides by theb-galactosidase fromEnterobacter cloacaeB5. Appl Microbiol Biotechnol. 2017, 101(12):4995−5003

12. Xiaodi Chen, Li Xu, Lan Jin, Bin Sun, Guofeng Gu, Lili Lu*,Min Xiao*.Efficient and regioselective synthesis of β-GalNAc/GlcNAc-lactose by a bifunctional transglycosylating β-N-acetylhexosaminidase fromBifidobacterium bifidum. Appl Environ Microbiol. 2016, 82(18):5642−5652.

13. Li Xu, Xiaohong Liu, Zhenhao Yin, Qian Liu, Lili Lu,Min Xiao*. Site-directed mutagenesis ofa-L-rhamnosidase fromAlternariasp. L1 to enhance synthesis yield of reverse hydrolysis based on rational design. Appl Microbiol Biotechnol. 2016, 100(24):10385−10394

14. Wei Gong，Li Xu，Guofeng Gu，Lili Lu，Min Xiao*. Efficient and regioselective synthesis of globotriose by a novela-galactosidase fromBacteroides fragilis. Appl Microbiol Biotechnol 2016,100(15): 6693–6702.

15. Shuquan Fan, Huaqin Zhang, Xiaodi Chen, Lili Lu, Li Xu andMin Xiao*. Cloning, characterization, and production of threea-L-fucosidases fromClostridium perfringensATCC 13124. J Basic Microbiol. 2015, 55(4):347−357.

16. Lili Lu, Qian Liu, Lan Jan, Zhenghao Yin, Li Xu,Min Xiao*. Enzymatic synthesis of rhamnose containing chemicals by reverse hydrolysis. PLoS One. 2015, 10(10):e0140531.

17. Lili Lu, Lijuan Xu, Yuchuan Guo, Dayu Zhang, Tingting Qi, Lan Jin, Guofeng Gu, Li Xu,Min Xiao*. Glycosylation of Phenolic Compounds by the Site-Mutated β-Galactosidase fromLactobacillus bulgaricusL3. PLoS ONE. 2015, 10(3): e0121445. doi:10.1371/journal.pone.0121445

18. Lili Zhang, Lili Lu, Shuquan Fan, Lan Jin, Guofeng Gu, Li Xu andMin Xiao*. One-step synthesis ofa-Gal epitope and globotriose derivatives by an engineereda-galactosidase. RSC Advances, 2015, 5(29), 22361 –22364

19. Li Xu, Dongxue Wang, Lili Lu, Lan Jin, Jiawei Liu, Deyong Song, Zhongwu Guo,Min Xiao*. Purification, Cloning, Characterization, and N-Glycosylation Analysis of a Novelb-Fructosidase fromAspergillus oryzaeFS4 Synthesizing Levan- and Neolevan-Type Fructooligosaccharides. PLoS ONE. 2014, 9(12): e114793.

20. Lili Lu, Feng Fu, Renfei Zhao, Lan Jin, Chunjuan He, Li Xu,Min Xiao*. A recombinant levansucrase fromBacillus licheniformis8-37-0-1catalyzes versatile transfructosylation reactions. Process Biochem. 2014, 49(9): 1503−1510

21. Lili Lu, Jian Wu, Deyong Song, Han Zhao, Guofeng Gu, Yuchuan Guo, Jin Lan,Min Xiao*. Purification of fructooligosaccharides by immobilized yeast cells and identification of ethyl β-d-fructofuranoside as a novel glycoside formed during the process. Bioresour Technol, 2013, 132:365–369

22. Jinhua Zhang, Lili Lu, Lijie Yin, Shen Xie,Min Xiao*. Carotenogenesis gene cluster and phytoene desaturase catalyzing both three- and four-step desaturations fromRhodobacter azotoformans. FEMS Microbiol Lett, 2012, 333(2):138–145.

23. Qian Liu, Lili Lu,Min Xiao*. Cell Surface Engineering of α-L-rhamnosidase for Naringin Hydrolysis. Bioresour Technol, 2012, 123:144–149.

24. Lili Lu, Shuze Xu, Renfei Zhao, Dayu Zhang, Zhengyi Li, Yumei Li,Min Xiao*. Synthesis of galactooligosaccharides by CBD fusion beta-galactosidase immobilized on cellulose. Bioresour Technol, 2012, 116:327−333

25. Lili Lu, Shuze Xu, Lan Jin, Dayu Zhang, Yumei Li,Min Xiao*. Synthesis of galactosyl sucralose by beta-galactosidase fromLactobacillus bulgaricusL3. Food Chem, 2012, 134(1):269–275

Patents