Yuhong Liua,, Yuguo Liu, Haiqiang Jiang, Lingchuan Xu, Yanna Cheng, Peng George Wang, Fengshan Wang
Carbohydrate Polymers 106 (2014) 42–48
Two sulfated derivatives (PRP-S1 and PRP-S2) of a -glucan from Phellinus ribis with different degrees ofsubstitution were obtained by chlorosulfonic acid method. The derivatives could block formation of newvessels in zebrafish and inhibit the proliferation of human umbilical vein endothelial cells (HUVECs).The two sulfated derivatives had remarkably high antitumor activities in vivo (in BALB/c mice inocu-lated with H22 hepatocellular carcinoma) as well as in vitro (against human ovary cancer SKOV-3 cells),without producing any overt signs of general toxicity. The results of immunohistochemistry assay indi-cated that the derivatives significantly reduced the average number of microvessel density (MVD) andinhibited the expression of vascular endothelial growth factor (VEGF) in tumor. Thus, these derivativesexhibit pronounced antiangiogenic and antitumoral properties. Except for cytotoxic effects on tumorcells, it is reasonable to expect that the antitumoral effects of PRP-S1 and PRP-S2 are mediated via theirantiangiogenic properties.