首页 > 新闻中心 > 文章速递
Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from glucose with elevated 3-hydroxyvalerate fraction via combined citramalate and threonine pathway in Escherichia coli
发表时间:2015-02-05 08:52:14

Qian Wang & Xiaolong Liu & Qingsheng Qi

Appl Microbiol Biotechnol (2014) 98:3923–3931

ABSTRACT

The biosynthesis of polyhydroxyalkanoate copolymers in Escherichia coli from unrelated carbon sources becomes attractive nowadays. We previously developed a poly(hydroxybutyrate-co-hydroxyvalerte) (PHBV) biosynthetic pathway from an unrelated carbon source via threonine metabolic route in E. coli(Chen et al.,Appl Environ Microbiol 77:4886-4893, 2011). In our study, a citramalate pathway was introduced in recombinant E. coli by cloning a cimAgene from Leptospira interrogans. By blocking the pyruvate and the propionyl-CoA catabolism and replacing the β-ketothiolase gene, the PHBV with 11.5 mol% 3HV fraction was synthesized. Further, the combination of citramalate pathway with the threonine biosynthesis pathway improved the 3HV fraction in PHBV copolymer to 25.4 mol%in recombinant E. coli.

Copyright 2012-2013 www.glycoeng.sdu.edu.cn All Rights Reserved. 山东大学国家糖工程技术研究中心 版权所有 技术支持:齐鲁八达

地址:山东省济南市山大南路27号  电话:0531-88363002  邮箱:glycoengineering@sdu.edu.cn